Staphylococcus aureus enterotoxin genes in nosocomial surfaces
Abstract
Enterotoxin-producing Sthapylococcus aureus is a pathogen that causes a large proportion of food poisoning worldwide and when found on nosocomial surfaces, can affect vulnerable patients, resulting in high morbidity and mortality rates that are difficult to control, especially in developing countries. We identified the presence of genes encoding for enterotoxins isolated from S. aureus strains on nosocomial surfaces in a health care institution. The research was qualitative, descriptive-cross-sectional, the population consisted of 200 samples obtained from surfaces from which six strains of S. aureus were isolated by phenotypic and genotypic methods; these were preserved in trypticase soy broth, then seeded on mannitol agar for reactivation and subsequent DNA extraction and finally the Polymerase Chain Reaction (PCR) was applied with specific primers for the detection of the genes sea, seb, sec, sed and see. 16.66% of the S. aureus strains were found to carry the sea gene and 16.66% the sed gene coding for enterotoxins of the same name, which is relevant since these genes are normally found in food.
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