The effect of Terebinth oil on Ezrin and Moesin expression levels in rats with ovarian ischemia-reperfusion Injury
Abstract
The objective of this study is to examine the expression levels of ezrin and moesin proteins from a molecular and immunohistochemical standpoint following the administration of terebinth oil in rats with ovarian ischemia- reperfusion injury. A total of 32 female Sprague-Dawley rats were utilized in the study. The rats were randomly assigned to one of four groups, with eight rats in each group: control, ischemia, I/R, and I/R+-terebinth oil. Following the induction of torsion, the treatment group received 2 ml.kg-1 of terebinth oil orally via gavage once daily for 28 d. At the conclusion of the experiment, ovarian tissues were obtained for immunohistochemical and molecular analysis. The immunohistochemical evaluation demonstrated a positive ezrin expression in epithelial cells within the I/R+-terebinth oil group, in comparison to the I/R group. Conversely, a negative reaction was observed in the vicinity of blood vessels. The expression of moesin was observed to be positive in granulosa cells and stromal areas. Additionally, a notable decline in the expression levels of ezrin and moesin proteins was observed in the treatment group in comparison to the damage group. Moreover, the administration of terebinth oil was observed to result in protein expression levels that were more closely aligned with those observed in the control group. The present study has demonstrated the impact of terebinth oil administration on the expression levels of ezrin and moesin proteins in a model of ovarian ischemia-reperfusion injury.
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Aktaş A, Aşır F, Özdemir Başaran S, Kaplan Ö, Ermiş IS, Deveci E. Granulocyte colony stimulating factor (GCSF) protected in ovarian tissues against ischemia reperfusion injury. JDDT. [Internet]. 2022; 12(4):26-30. doi: https://doi.org/nh3h DOI: https://doi.org/10.22270/jddt.v12i4.5538
Demir Çaltekin M, Özkut MM, Çaltekin I, Kaymak E, Çakır M, Kara M, Yalvaç ES. The protective effect of JZL184 on ovarian ischemia reperfusion injury and ovarian reserve in rats. J. Obstet. Gynaecol. Res. [Internet]. 2021; 47(8):2692-2704. doi: https://doi.org/gj4gtw DOI: https://doi.org/10.1111/jog.14859
Colak S, Gurlek B, Topcu A, Tumkaya L, Mercantepe T, Yilmaz A. Protective effects of nebivolol on ovarian ischemia–reperfusion injury in rat. J. Obstet. Gynaecol. Res. [Internet]. 2020; 46(11):2407-2416. doi: https://doi.org/nh3j DOI: https://doi.org/10.1111/jog.14503
Kaplan S, Türk A. Effects of vitamin B12 on rat ovary with ischemia-reperfusion injury. Biotech Histochem. [Internet]. 2022; 97(4):284-289. doi: https://doi.org/gr8tkf DOI: https://doi.org/10.1080/10520295.2021.1961863
Güvenç M, Yüksel M, Kutlu T, Etyemez M, Gökçek İ, Cellat M. Protective effects of esculetin against ovary ischemia-reperfusion injury model in rats. J Biochem Mol Toxicol. [Internet]. 2024; 38(1):e23528. doi: https://doi.org/nh3k DOI: https://doi.org/10.1002/jbt.23528
Grace PA. Ischaemia-reperfusion injury. Br J Surg. [Internet]. 1994; 81(5):637-47. doi: https://doi.org/cbmzvs DOI: https://doi.org/10.1002/bjs.1800810504
Xie JJ, Zhang FR, Tao LH, Lü Z, Xu XE, Jian-Shen, Xu LY, Li EM. Expression of Ezrin in Human Embryonic, Fetal, and Normal Adult Tissues.J Histochem Cytochem. [Internet]. 2011; 59(11):1001-1008. doi: https://doi.org/b6j8hg DOI: https://doi.org/10.1369/0022155411418661
Xu WT, Shi LL, Xu J, Qian H, Zhou H, Wang LH. Ezrin expression in female reproductive tissues: A review of regulation and pathophysiological implications. Front. Cell dev. biol. [Internet]. 2023; 11:1-9. doi: https://doi.org/nr89 DOI: https://doi.org/10.3389/fcell.2023.1125881
Maniti O, Carvalho K, Picart C. Model membranes to shed light on the biochemical and physical properties of ezrin/radixin/moesin. Biochimie. [Internet]. 2013; 95(1):3-11. doi: https://doi.org/f4h26z DOI: https://doi.org/10.1016/j.biochi.2012.09.033
Furthmayr H, Lankes W, Amieva M. Moesin, a new cytoskeletal protein and constituent of filopodia: Its role in cellular functions. Kidney Int. [Internet]. 1992; 41(3):665-670. doi: https://doi.org/b56bdb DOI: https://doi.org/10.1038/ki.1992.102
Amieva MR, Furthmayr H. Subcellular Localization of Moesin in Dynamic Filopodia, Retraction Fibers, and Other Structures Involved in Substrate Exploration, Attachment, and Cell-Cell Contacts. Exp. Cell Res. [Internet]. 1995; 219(1):180-196. doi: https://doi.org/cx2926 DOI: https://doi.org/10.1006/excr.1995.1218
Tuzlaci E, Aymaz PE. Turkish folk medicinal plants, part IV: Gonen (Balikesir). Fitoterapia. [Internet]. 2001; 72(4):323-343. doi: https://doi.org/d77fz8 DOI: https://doi.org/10.1016/S0367-326X(00)00277-X
Hayoğlu İ, İzol G, Gümüş A, Göncü B, Çevik GB. Menengicin şekerleme üretiminde kullanım olanakları. J. Agric. Fac. Harran Univ. [Internet]. 2010 [Cited 24 june 2024]; 14(4):57-62. Available in: https://n9.cl/hbv7a
Özcan M. Characteristics of fruit and oil of terebinth (Pistacia terebinthus L) growing wild in Turkey. J. Sci. Food. Agric. [Internet]. 2004; 84(6):517-520. doi: https://doi.org/bc7bfh DOI: https://doi.org/10.1002/jsfa.1632
Erısır FE, Kıreccı OA, Yılmaz O, Özsahın AD. Effects of Pistacia terebinthus L. Subsp. palaestina and Rhus coriaria L. Plants on Some Biochemical Parameters of Brain Tissue of Sprague-Dawley Rats in Experimental Breast Cancer Model. KSU J. Agric. Nat. [Internet]. 2022; 25(1)33-41. doi: https://doi.org/nh3m DOI: https://doi.org/10.18016/ksutarimdoga.vi.1074615
Şındak N, Akgül MB, Gülaydın A, Karakoç Z. Effects of Topical Terebinth Berry Oiland Different Experimental Mixtures on Wound Healing in Japanese Quails (Coturnix Coturnix Japonica). Van. Vet. J. [Internet]. 2017 [Cited 12 July 2024]; 28(2):69-74. Available in: https://n9.cl/t0na0
Uyar A, Abdulrahman NT. A histopathological, immuno-histochemical and biochemical investigation of the antidiabetic effects of the Pistacia terebinthus in diabetic rats. Biotech. Histochem. [Internet]. 2020; 95(2):92-104. doi: https://doi.org/nh3p DOI: https://doi.org/10.1080/10520295.2019.1612092
Eser A, Hızlı D, Haltas H, Namuslu M, Kosus A, Kosus N, Kafalı H. Effects of curcumin on ovarian ischemia-reperfusion injury in a rat model. Biomed Rep. [Internet]. 2015; 3(6):807-813. doi: https://doi.org/nh3q DOI: https://doi.org/10.3892/br.2015.515
Kawaguchi K, Yoshida S, Hatano R, Asano S. Pathophysiological Roles of Ezrin/Radixin/Moesin Proteins. Biol. Pharm. Bull. [Internet]. 2017; 40(4):381–390. doi: https://doi.org/f93hxx DOI: https://doi.org/10.1248/bpb.b16-01011
Louvet S, Aghion J, Santa-Maria A, Mangeat P, Maro B. Ezrin Becomes Restricted to Outer Cells Following Asymmetrical Division in the Preimplantation Mouse Embryo. [Internet]. Dev. Biol. 1996;177(2):568-79. doi: https://doi.org/cxzhp8 DOI: https://doi.org/10.1006/dbio.1996.0186
Wakayama T, Nakata H, Kurobo M, Sai Y, Iseki S. Expression, Localization, and Binding Activity of the Ezrin/Radixin/Moesin Proteins in the Mouse Testis. J. Histochem. Cytochem. [Internet]. 2009; 57(4):351-362. doi: https://doi.org/dcpqpm DOI: https://doi.org/10.1369/jhc.2008.952440
Baiocchi L, Tisone G, Russo MA, Longhi C, Palmieri G, Volpe A, Almerighi C, Telesca C, Carbone M, Toti L, De Leonardis F, Angelico M. TUDCA prevents cholestasis and canalicular damage induced by ischemia-reperfusion injury in the rat, modulating PKCa–ezrin pathway. Transpl. Int. [Internet]. 2008; 21(8):792-800. doi: https://doi.org/ dbps8v DOI: https://doi.org/10.1111/j.1432-2277.2008.00682.x
Sak ME. Gestasyonel Diabet Olan ve Olmayan Hastaların Plasentalarında Ezrin Radixin-Moesin Proteinlerinin İmmunohistokimya ve Western Blot Yöntemleriyle Expresyonlarının Değerlendirilmesi [Doctoral thesis on the In-ternet]. Dicle University; 2020 [Cited 18 Sep 2024]. 54 p. Available in: https://n9.cl/h8f6wv
Ağaçayak E. Sigara İ çen ve İçmeyen Preeklamptik Plasentalarda Ezrin-Radixin Moesin Protein Expresyonlarının İmmünohistokimyasal ve Western Blot Yöntemiyle Değerlendirilmesi [Doctoral thesis on the Internet]. Dicle University; 2020 [cited 18 Sep 2024]. 80 p. Available in: https://n9.cl/qfedf
Fadiel A, Chen Z, Ulukus E, Ohtani K, Hatami M, Naftolin F. Ezrin Overexpression by Transformed Human Ovarian Surface Epithelial Cells, Ovarian Cleft Cells, and Serous Ovarian Adenocarcinoma Cells. Reprod. Sci. [Internet]. 2012;19(8):797-805. doi: https://doi.org/f3436h DOI: https://doi.org/10.1177/1933719111433884
Li M.J, Xiong D, Huang H, Wen ZY. Ezrin Promotes the Proliferation, Migration, and Invasion of Ovarian Cancer Cells. Biomed. Environ Sci. [Internet]. 2021; 34(2):139 151. doi: https://doi.org/nh3w
Topçu G, Ay M, Bilici A, Sarıkürkcü C, Öztürk M, Ulubelen A. A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chemistry. [Internet]. 2007; 103(3)816–822. doi: https://doi.org/cbfc3g DOI: https://doi.org/10.1016/j.foodchem.2006.09.028
Tastekin D, Tambas M, Kilic K, Erturk K, Arslan D. The efficacy of Pistacia terebinthus soap in the treatment of cetuximab-induced skin toxicity. Invest New Drugs. [Internet]. 2014; 32(6):1295-300. doi: https://doi.org/f6qk84 DOI: https://doi.org/10.1007/s10637-014-0128-z
Altunova H. Menengiç (Pistacia terebinthus L.) Meyve Ekstresinin Metisiline Dirençli Staphylococcus aureus (MRSA) ve Vankomisine Dirençli Enterococcus (VRE)’a Karşı Antibakteriyel Aktivitesi [Master’s thesis on the Internet]. Gaziantep University; 2016 [cited 18 Sep 2024]. 60 p.
Ozsahin AD, Erdem Erisir F, Tuzcu Z.. Influences of Pistacia terebinthus L. and Rhus coriaria L. fruits extracts on the levels of some biochemical parameters in liver tissue of Experimental Breast Cancer rats. Progr Nutr[Internet]. 2021; 23(2): e2021063. doi: https://doi.org/nh3t
