Production of extracellular chitinase from Serratia marcescens QM B1466 using chitin from shrimp shells waste
Abstract
Chitin from shrimp shells was prepared to evaluate the production of extracellular chitinase from Serratia marcescens QM B1466 in submerged culture under controlled conditions of temperature (28°C). shaking (150 rpm) and pH (7.7).To determine the composition of chitin on the shell, the material was ground, classified by sieving, deproteinized and demineralized. The proximate waste analysis indicates a high content of crude protein (36.11%). The highest chitinase activity during the enzymatic hydrolysis process was 1.91 µmols. equivalent to N-actetylglucosamine produced per mi of filtered culture: this determines the enzyme capacity of hydrolysis on the crystalline chitin. The percentage of chitin conversion during fermentation (73%) is the result of this activity. The N-acetylglucosamine produced is used by the bacterium for growth. which was adjusted to the Monod model.
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