Effect of melatonin and melatonin plus progestagen protocols on some reproductive performance and blood parameters at the beginning of the breeding season in Hasmer ewes
Abstract
The aim of this study was to investigate the effect of exogenous melatonin administered by different methods at the beginning of the breeding season on estrus onset, fertility, plasma progesterone and malondialdehyde (MDA) concentrations in Hasmer ewes. To this end, the ewes were divided into three groups: Group I (MEL): This group received subcutaneously 18 mg melatonin implant (RegulinTM, Ceva– Turkiye) Group II (MELPRO): This group received subcutaneously 18 mg of melatonin implant (RegulinTM, Ceva–Turkiye), followed by the intravaginal administration of a sponge containing 20 mg of flugestone acetate (Choronogest CRTM–France) for a period of 9 day. This was followed by the intramuscular injection of 250 µg of cloprostenol (SenkrodinTM– Vetas–Turkiye) as soon as the sponge was removed. Group III designated as the control group (CON), received 1 mL of saline was administered subcutaneously for placebo effect. Estrus was detected using teaser rams starting 24 h after the melatonin (Group MEL), PGF2α (Group MELPRO) and physiological saline (Group CON) applications twice a day (06:00 and 17:00). Ewes in oestrus were moved to separate compartments and mated with fertile rams. Blood samples were collected from the vena jugularis on the day of treatment initiation, on the day of mating, and on day 17 of gestation. Pregnancy examinations were performed between 25 and 30 days after mating, and heartbeats were monitored using a 5–8 MHz linear trans–rectal probe in the groups (DP 50 VETTM, Mindray Ltd. China). There was no statistical difference in the onset of first oestrus between the groups (P>0.05). A noteworthy finding was the increase in progesterone concentration on the 17th day of pregnancy in the group treated with melatonin alone, compared to other groups, at the beginning of the breeding season (P<0.05).
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Tamura H, Nakamura Y, Terron MP, Flores LJ, Manchester LC, Tan D, Sugino S, Reíter RJ. Melatonin and pregnancy in the human. Reprod. Toxicol. [Internet]. 2008; 25(3):291–303. doi: https://doi.org/c6dcxj
Atasoy ÖB, Erbaş O. Physiological effects of the melatonin hormone. Ist. Bilim Üniv. Florence Nightingale Tip. Derg. [Internet]. 2017 [cited 12 Nov. 2024]; 3(1):52–62. Available in: https://goo.su/JncWxc
Tsutsui K, Ubuka T. Discovery of gonadotropin–inhibitory hormone (GnIH), progress in GnIH research on reproductive physiology and behavior and perspective of GnIH research on neuroendocrine regulation of reproduction. Mol. Cell. Endocrinol. [Internet]. 2020; 514:110914. doi: https://doi.org/pb6m
Balík A, Kretschmannova K, Mazna P, Svobodova I, Zemkova H. Melatonin action in neonatal gonadotrophs. Physiol. Res. [Internet]. 2004; 53(Suppl. 1):S153–S166. doi: https://doi.org/pb6n
Ferlazzo N, Andolina G, Cannata A, Costanzo MG, Rizzo V, Currò M, Lentile R, Caccamo D. Is melatonin the cornucopia of the 21st century?. Antioxidants [Internet]. 2020; 9(11):1088. doi: https://doi.org/gmgbbz
Vlckova R, Posivak J, Valocký I, Sopkov, D, Andrejcakova, Z, Kostecka Z, Kozioł K, Seidavi A. Effects of short–term melatonin or progestogen with gonadotropic treatments on reproductive performance, hormonal levels and ovarian activity of ewes. Int. J. Vet. Sci. [Internet]. 2022; 11(4):493–497. doi: https://doi.org/pb6p
Xiao L, Hu J, Song L, Zhang Y, Dong W, Jiang Y, Zhang Q, Yuan L, Zhao X. Profile of melatonin and its receptors and synthesizing enzymes in cumulus–oocyte complexes of the developing sheep antral follicle—a potential estradiol– mediated mechanism. Reprod. Biol. Endocrinol. [Internet]. 2019; 17:1. doi: https://doi.org/pb6s
Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N. Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J. Pineal Res. [Internet]. 2008; 44(3):280–287. doi: https://doi.org/d6bgp7
Vazquez MI, Forcada F, Sosa C, Casao A, Sartore I, Fernández– Foren A, Meikle A, Abecia JA. Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes. Anim. Reprod. Sci. [Internet]. 2013; 141(1–2):52–61. doi: https://doi.org/f49w4t
Tian X, Wang F, He C, Zhang L, Tan D, Reiter RJ. Xu J, Ji P, Liu G. Beneficial effects of melatonin on bovine oocytes maturation: a mechanistic approach. J. Pineal Res. [Internet]. 2014; 57(3):239–247. doi: https://doi.org/f6hhr6
Şener G. Karanlığın hormonu: Melatonin [Hormone of darkness: Melatonin]. Marmara Pharm. J. [Internet] 2010 [cited 22 Oct. 2024]; 14:112–120. Turkish. Avaliable in: https://goo.su/AEsSh
Nagel C, Aurich C, Aurich J. Stress effects on the regulation of parturition in different domestic animal species. Anim. Reprod. Sci. [Internet]. 2019; (207):153–161. doi: https://doi.org/pb6x
Akbulut NK, Kal Y. Effects of tocolytic drugs (isoxsuprine hydrochloride) during the implantation period in pregnant sheep. Pol. J. Vet. Sci. [Internet]. 2022; 25(4):557–560. doi: https://doi.org/pb6w
Magarelli PC, Cridennda DK, Cohen M. Changes in serum cortisol and prolactin associated with acupuncture during controlled ovarian hyperstimulation in women undergoing in vitro fertilization–embryo transfer treatment. Fertil. Steril. [Internet]. 2009; 92(6):1870–1879. doi: https://doi.org/db6qnf
Wallace JM, Robinson JJ, Wigzell S, Aitken RP. Effect of melatonin on the peripheral concentrations of LH and progesterone after oestrus, and on conception rate in ewes. The J. Endocrinol. [Internet]. 1988; 119(3):523–530. doi: https://doi.org/bcmnxk
Abecia J, Forcada F, Zuniga O. The effect of melatonin on the secretion of progesterone in sheep and on the development of ovine embryos in vitro. Vet. Res. Commun. [Internet]. 2002; (26):151–158. doi: https://doi.org/fws46h
Özyurtlu N, Bademkıran S. Koyunlarda östrus senkronizasyonu ve östrusu uyarma yöntemleri [Estrus synchronization and ınduction of estrus methods in sheep]. Dicle Univ. Vet. Fak. Derg. [Internet]. 2010 [cited 1 Dec. 2024]; 1(1):17–22. Turkish. Available in: https://goo.su/50VLL
Fernandez J, Bruno–Galarraga MM, Sot AT, de la Sota RL, Cueto MI, Lacau IM, Gibbons E. Hormonal therapeutic strategy on the induction of accessory corpora lutea in relation to follicle size and on the increase of progesterone in sheep. Theriogenology [Internet]. 2018; 105:184–188. doi: https://doi.org/pb8v
Buege JA, Aust SD. Microsomal lipid peroxidation. In: Fleischer S, Packer L, editors. Methods in Enzimology [Internet]. Cambridge (MA, USA): Academic Press; 1978. p. 302–310. (Vol. 52). doi: https://doi.org/cgmt6k
Kutlu M, Doğan H, Durmuş, M. The relationship of anogenital distance with fertility and anti–müllerian hormone in Awassi and Cukurova meat ewes. Small Rumin. Res. [Internet]. 2024; 241:107390. doi: https://doi.org/pb8w
International Business Machines Corporation (IBM). Released 2015. IBM SPSS Statistics for Windows, Version 23.0 [Internet]. Armonk (NY, USA): IBM Corp; 2015 [cited 1 Dec. 2024]. Available in: https://goo.su/RTzscym
Zarazaga LA, Gatica M, Celi I, Guzman J, Malpaux B. Artificial long days in addition to exogenous melatonin and daily contact with bucks stimulate the ovarian and oestrous activity in Mediterranean goat females. Animal [Internet]. 2011; 5(9):1414–1419. doi: https://doi.org/fkdznz
Kusakari N, Ohara M. Effect of melatonin feeding on early onset of reproductive activity in postpartum Suffolk ewes lactating during anestrous season. J. Reprod. Develop. [Internet]. 1997; 43(1):97-100. doi: https://doi.org/fwqmrq
Guan S, Xie L, Ma T, Lv D, Jing W, Tian X, Song Y, Liu Z, Xiao X, Liu G. Effects of Melatonin on Early Pregnancy in Mouse: Involving the Regulation of StAR, Cyp11a1, and Ihh Expression. Int. J. Mol. Sci.. [Internet]. 2017; 18:1637. doi: https://doi.org/gbxzbz
Zhang L, Zhang Z, Wang F, Tian X, Ji P, Liu G. Effects of melatonin administration on embryo implantation and offspring growth in mice under different schedules of photoperiodic exposure. Reprod. Biol. Endocrinol. [Internet]. 2017; 15:78. doi: https://doi.org/gb2q4z
Haresign W. The effect of implantation of lowland ewes with melatonin on the time of mating and reproductive performance. Anim. Sci. [Internet]. 1992; 54(1):31-39. doi: https://doi.org/fj8j4d
Leyva–Corona JC, Angulo–Valenzuela NI, Laborin–Escalante BM, Gastelum–Delgado MA, Silva–Avila NJ, Luna–Nevárez P, Aragón–López CE, Sánchez–Castro MA, Morales–Pablos MI. Reproductive performance of hair ewes and rams implanted with Melatonin previous to the anestrus season in Northwest Mexico. Trop. Anim. Health Product. [Internet]. 2023; 55(3):174. doi: https://doi.org/pb84
Abd–Allah M, Daghash MWH. The role of exogenous melatonin and photoperiod on productive and reproductive performance of Ossimi sheep. Egypt. J. Sheep Goats Sci. [Internet]. 2019[cied Dec 13 2024]; 14(3):49-58. Available in: https://goo.su/royIn
Fang L, Li Y, Wang S, Yu Y, Li Y, Guo Y, Yan Y, Sun YP. Melatonin induces progesterone production in human granulosa–lutein cells through upregulation of StAR expression. Aging [Internet]. 2019; 11(20):9013-9024. doi: https://doi.org/pb89
Taketani T, Tamura H, Takasaki A, Lee L, Kizuka F, Tamura I, Taniguchi K, Maekawa R, Asada H, Shimamura K, Reiter RJ, Sugino N. Protective role of melatonin in progesterone production by human luteal cells. J. Pineal Res. [Internet]. 2011; 51(2):207-213. doi: https://doi.org/c7h8kb
Ma J, Wang J, Hu S, Li Y, Zhang Y, Yang Y, Yang C, Huo S, Yang Y, Zhaxi Y, Luo W. Effects of melatonin on development and hormone secretion of sheep theca cells in vitro. Theriogenology [Internet]. 2023; 198:172-182. doi: https://doi.org/pb9b
Horoz H, Kaşıkçı G, Ak K, Alkan S, Sönmez C. Controlling the breeding season using melatonin and progestagen in Kıvırcık ewes. Turk. J. Vet. Anim. Sci. [Internet]. 2003 [cited 19 Nov. 2024]; 27(2):301-305. Available in: https://goo.su/qV2S1
Fan W, He Y, Guan X, Gu W, Wu Z, Zhu X, Huang F, He H. Involvement of the nitric oxide in melatonin–mediated protection against injury. Life Sci. [Internet]. 2018; 200:142-147. doi: https://doi.org/gdd6c8
Morvaridzadeh M, Sadeghi E, Agah S, Nachvak SM, Fazelian S, Moradi F, Persad E, Heshmati J. Effect of melatonin supplementation on oxidative stress parameters: a systematic review and meta–analysis. Pharmacol. Res. [Internet]. 2020; 161:105210. doi: https://doi.org/gr5szn
El–Sawi MR, Badawy ME, El–Gharieb NM. Role of melatonin in modulation of oxidative stress induced by delta–aminolevulinic acid in adult male albino rats. Egypt. J. Hosp. Med. [Internet]. 2007; 28(1):233-272. doi: https://doi.org/pb9c
Pertsov SS, Koplik EV, Kalinichenko LS, Alekseeva IV. Effects of melatonin on lipid peroxidation in blood in rats with different behavioral characteristics in acute emotional stress. Neurosci. Behav. Physiol. [Internet]. 2016; 46:133-137. doi: https://doi.org/pb9d
Pasqualotto EB, Agarwal A, Sharma RK, Izzo VM, Pinotti JA, Joshi NJ, Rose B. Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures. Fertil Steril. [Internet]. 2004; 81(4):973-976. doi: https://doi.org/dj3dpv
